Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome
نویسندگان
چکیده
منابع مشابه
Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome.
The nucleotide sequence of the DNA genome of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV), a group II NPV, was determined and analysed. The genome contains 135611 bp and has a G+C content of 44 mol%. Computer-assisted analysis revealed 139 ORFs of 150 nucleotides or larger; 103 have homologues in Autographa californica MNPV (AcMNPV) and a further 16 have homologues in other bacul...
متن کاملA New Continuous Cell Line of Spodoptera exigua and Its Susceptibility to Autographa californica Multicapsid Nucleopolyhedrovirus
A continuous cell line, designated KU-SENL-1 (SENL1), has been established from minced neonate larvae of Spodoptera exigua (Lepidoptera: Noctuidae) treated with collagenase. The primary culture was maintained in TC100 medium supplemented with 10% Fetal Bovine Serum (FBS), 3% Helicoverpa armigera hemolymph and incubated at 27°C. This continuous cell line was cultured in TC100 medium supplemented...
متن کاملSequence and transcriptional analysis of the ubiquitin gene cluster in the genome of Spodoptera exigua nucleopolyhedrovirus.
The nucleotide sequence of a 1200 bp DNA fragment of Spodoptera exigua nucleopolyhedrovirus (SeMNPV) was determined. This sequence contained a cluster of two open reding frames (ORFs), one coding for a viral ubiquitin (v-ubi) and another with homology to orf2 of Autographa californica (Ac) MNPV and Orgyia pseudotsugata (Op) MNPV. The vubi ORF is 240 nucleotides (nt) long, potentially encoding a...
متن کاملCharacterization of Spodoptera exigua multicapsid nucleopolyhedrovirus ORF17/18, a homologue of Xestia c-nigrum granulovirus ORF129.
Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) contains a number of genes with a homologue found so far only in a distantly related baculovirus. One of these, SeMNPV ORF17/18 (Se17/18) shares 55% amino acid similarity to ORF129 of Xestia c-nigrum granulovirus (XcGV). Se17/18 was transcribed in cultured S. exigua 301 cells, as a polyadenylated transcript of 1.1 kb. 5'-RACE analysis ...
متن کاملInteractions between Meteorus pulchricornis and Spodoptera exigua Multiple Nucleopolyhedrovirus
Baculoviruses may interact with parasitoids in the same host. A previous study has shown that infection of larvae with Spodoptera litura nucleopolyhedrovirus (SpltNPV) was deleterious to the survival and development of Meteorus pulchricornis (Wesmael) (Hymenoptera: Braconidae). In this paper, the interactions between M. pulchricornis and Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1999
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-80-12-3289